Tulane
National Primate Research Center
Tulane
Annual Progress Report 2003-2004
The Tulane National Primate Research Center competes
with the Southwest Foundation for Biomedical Research for the dubious
"honor" of imprisoning the largest monkey population among
the eight National Institutes of Health's Regional Primate Research
Centers. Tulane has over 4,500 monkeys of eleven species. Rhesus
macaques form the overwhelming majority with at least 3,500 on hand.
A drive through the facility offers a rare glimpse of caged research
monkeys with only a hedge and a barbed-wire fence separating acres
of pens from the road.
In mid October of 1998, two dozen rhesus monkeys escaped from cages
in this outdoor area. Tulane officials were quick to assert that
the monkeys were not infected with any disease and posed no risk
to the public. But consider the Blanchard report below. At least
70% of the TRPRC's monkeys are infected with herpes-B, a disease
fatal to humans. This disease has been used as a club against macaques
for some time. In Wisconsin, primate center officials used the high
incidence figures (common in all captive macaque colonies subjected
to regular high stress) to argue that the macaques at the county
zoo were a grave risk to the public's health and could not be safely
maintained on public display. More recently when rhesus monkeys
escaped from an island in Florida, Kirk Boehm, a Wisconsin primate
center minor official, once again publicly asserted that macaques
pose a risk to the public. The NIH RPRC officials want it both ways.
When the public is worried, the monkeys pose no risk; when the public
is concerned for the monkeys' well-being, the monkeys are too dangerous
to have around. This is the mentality and logic consuming our limited
tax dollars and subjecting thousands of monkeys annually to grievous
suffering.
Many
of the the
experiments being funded by the National Institutes of Health conducted
at the TNPRC are listed below. Most researchers (e-mail addresses
provided) are conducting many studies at any one time.
As with other very large primate experimentation sites, it is impossible
to keep completely up to date on all the experiments taking place.
This snapshot was compiled at the beginning of 2002.
BAGBY, GREGORY J. "Ethanol suppression of this response
was similar in cells from both control and SIV-infected animals.
These results suggest that alcohol might cause a further impairment
of host defense in SIV-infected animals and result in an earlier
and/or increased incidence of opportunistic infections in these
animals....The close similarities between HIV disease in people
and SIV infection in rhesus monkeys, together with out initial findings,
demonstrate the appropriateness of the rhesus monkey SIV model for
the study of the potential impact of alcohol on HIV/AIDS."
If the "close similarities between HIV disease in people and
SIV infection in rhesus monkeys" had really been demonstrated
or were menaingful, the tens of thousands of monkeys killed from
experimental SIV infection would have led to some benefit to humans
with HIV by now, but it hasn't. Bagby's initial findings are meaningless,
as is demonstrated by the relative dearth of other researchers around
the country using the same model.
BASKIN, GARY B. (a senior veterinarian) <gbask@tpc.tune.edu>
writes: "The objectives of this grant are to maintain and propagate
a colony of rhesus monkeys that are carriers of globoid cell leukodystrophy
(GLD) and to characterize GLD as it occurs in homozygous affected
individuals. The long-term goal of this project is to develop a
nonhuman primate animal model for gene therapy of inborn errors
of metabolism. This colony of rhesus monkeys is the only colony
of nonhuman primates in the world in which an inherited lysosomal
disorder has been recognized, propagated, and is available for study.
No other nonhuman primate model is available in which to test the
actual clinical effectiveness of various therapeutic interventions
in animals with a genetic disease." Baskin has explained, "An
infant rhesus monkey with this disease was diagnosed at TRPRC in
1989. Since then, we intentionally inbred this group of animals
and in 1996 observed two additional affected infants."
GLD is also called Krabbe's disease. Baskin studiously fails to
mention that the disease is well-known in West Highland White Terriers
and Cairn Terriers. We wonder whether he believes monkeys are good
models of dog diseases.
GLD is a rare disease in humans. Baskin also fails to mention that
human clinical studies are underway. Clearly, studying and trying
to cure humans with the disease is much more likely to lead to a
cure for humans than trying to create and study the disease in an
alien species. Baskin also fails to mention the suffering that his
breeding scheme will create. Symtoms may include loss of previously
attained developmental skills, fevers, irritability, myoclonic seizures
(sudden shock-like contractions of the limbs), blindness, spasticity
(stiffness of the limbs), and paralysis. Prolonged weight loss may
occur also. Onset of the disorder in humans is generally between
3 and 6 months of age.
BEILKE, MARK A. <mabeilke@tulane.edu>
or <idgeno@tulane.edu>
"This collaborative project established an animal model of
HTLV-I associated disease in nonhuman primates with a primary HTLV-I
viral isolate, derived from a human patient with HTLV-I associated
tropical spastic paraparesis (TSP). Two of three inoculated rhesus
macaques became infected and showed signs of disease. One animal
presented with a findings identical to that in the original patient,
with muscle atrophy, polymyositis, and arthritis, (manuscript published).
The second animal was dually infected with SIV and developed signs
suggestive of smoldering HTLV-I induced leukemia in the presence
of SIV immunodeficiency disease. Four of five additional HTLV-I
inoculated animals are asymptomatic but show evidence of persistent
infection by HTLV-I PCR and increased lymphocytosis. The fifth animal
is culture and PCR negative with an associated strong humoral response,
as determined by western blot analysis."
BLANCHARD, JAMES L. (Executive Director) <bubba@tpc.tulane.edu>
"The single specific aim of this application is to establish
a colony of Indian rhesus monkeys, free of Simian Immunodeficiency
Virus (SIV), simian retrovirus (SRV), herpes b virus, and simian
t-lymphotrophic virus (STLV 1). This will be accomplished by recruitment
of 100 specific pathogen free (SPF) female rhesus monkeys and 15
SPF males in the first year. An additional 50 SPF females and 10
males will be added to the founding colony in each of the subsequent
4 years. We project that by the end of the grant period the colony
will have 625 animals."
RUDOLF P BOHM, JR <bohm@tpc.tulane.edu>
"This study is a continuation of previously reported work
to develop a rhesus monkey model of Pneumocystis carinii pneumonia
(PCP). This model will be important to study the pathogenesis and
novel drug compounds that may be used to treat PCP. Four rhesus
monkeys were chosen for this pilot study. Three animals received
alternate day dosing of prednisone and cyclophosphamide to induce
immunosuppression. The fourth animal was used as a non-immunosuppressed
control. Bronchoalveolar lavage (BAL) was performed one week prior
to immunosuppression and weekly thereafter. All four animals were
inoculated via a bronchoscope with a homogenate from P. carinii
frozen lung tissue derived from three SIV infected animals."
COGSWELL, FRANK B. <cogswel@tpc.tulane.edu>
"Cerebral malaria, a syndrome found in patients infected with
Plasmodium falciparum, kills an estimated 2 million children a year.
The lack of an animal model has been a barrier to research into
supportive therapies and treatment."
Frank is profoundly ignorant of the problems associated with health
care in the developing world where malaria is common. In these regions,
most who contract malaria receive no treatment whatsoever.
His claim that the rate of death among children is linked to "the
lack of an animal model" is indicative of life in a very lofty
ivory tower.
Cogswell continues: "We are developing a model of this condition
in rhesus monkeys inoculated with P. knowlesi, a simian malaria
parasite....We have now selected a population of parasites that
consistently adhere to brain endothelium and we will next put these
parasites back into a rhesus monkey to produce cerebral malaria.
Once this sydrome has been seen in the experimental animals, we
will be able to test treatments and supportive therapies that can
be taken to endemic areas for use in children afflicted with this
condition."
Hey Frank, the children in these areas don't have access to the
simplest theraputics, like aspirin or clean water. Why not start
with the known treatments and cures before trying to invent more
therapies that the kids won't get? Does it have anything to do with
money?
CUSICK, CATHERINE G. <cusick@tulane.edu>
"The long term goal of this research is to understand the neural
substrates of certain forms of complex visual behavior and other
higher cortical functions. To this end, the proposed studies will
examine the functional organization of the superior temporal polysensory
area (STP) in the superior temporal sulcus (STS) of the rhesus monkey."
Cusick plans to place electrodes in the monkeys' brains, shock them
to see what happens to their eyes, then inject chemicals into their
brains and try to figure out how their brain controls their eyes.
DAVISON, BILLIE B. <billie@tpc.tulane.edu>
"In studies at TRPRC utilizing simian immunodeficiency virus
(SIV)-infected pregnant macaques, chorioamnionitis (CA) [Infection,
of the chorionic and amniotic membranes caused by bacteria.] and/or
increased inflammatory infiltrates within placental tissues was
associated with increased SIV antigen within placental tissues,
early fetal demise, and SIV transmission to the infant. During year
one (1998-99), this project will develop a primate model of chorioamnionitis
by introducing 2 different species and 4 doses of bacteria into
the choriodecidual space during pregnancy. During years 2-5, CA
will be induced in a group of SIV-infected monkeys and the transmission
outcome in this group will be compared to a group of monkeys infected
with SIV alone to directly test the hypothesis that increases in
inflammatory cell infiltrates within placental tissues increase
the rate of transmission of immunodeficiency virus from mother to
infant."
Besides being simply cruel, Davison appears to be just simple.
SIV (simian immunovirus), like HIV, is a disease that damages the
immune system. Chorioamnionitis is a bacterial infection. If a bacterial
infection has become established, the victim's immune system must
be compromised. It can be no surprise that when ill, it is easier
to become even more sick when exposed to another pathogen or that,
when additionally infected, that the immune system will be further
incapacitated. And, his hypothesis is meaningless as well. Few HIV-positive
pregnant women can forsee, and thus prevent, acquiring a bacterial
infection of the amniotic sack.
In another publicly-funded study, this time of malaria's impact
on babies, Davison claims, "The information derived from these
studies will allow [not might allow, or could allow, but will
allow] effective interventions to be designed which will prevent
[there's that certainty again] the devastating effects of malaria
in pregnant women and their children." Obviously, Davison believes
he can see into the future. In this series of monstrous experiments,
Davison will: "[D]etermine the effects of parity and prior
exposure to Plasmodium [the malaria causing organism] on the course,
severity, and outcome of clinical malaria in the mother, fetus,
and newborn and to test the hypothesis that macrophage-induced cytokine
imbalance causes morphologic and physiologic placental lesions that
result in fetal damage, post-natal failure to thrive, and congenital
infection."
DIDIER, ELIZABETH S. <esdid@tpc.tulane.edu>
"Microsporidia cause opportunistic infections in persons with
AIDS, organ transplant recipients, children, and travelers. Enterocytozoon
bieneusi is the most prevalent microsporidian but attempts to establish
a tissue culture system for generating organisms has been unsuccessful.
The only nonhuman hosts known for E. bieneusi include pigs and nonhuman
primates (eg. Macaca mulatta). Ten SIV-infected rhesus macaques
were inoculated orally with E. bieneusi harvested from the stool
and duodenal lavage aspirates of human AIDS patients. Spores were
detected in stools one week later and continued sporadically for
approximately two years or until death of the monkeys, but the inconsistency
of spore shedding presently renders this model inadequate for testing
antimicrosporidial compounds. However, the monkeys did become infected
with E. bieneusi and parasite-associated lesions were identified
in the gall bladder, liver, and small intestine. Attempts to infect
small animals (eg. gerbils, at hymic mice, immunesuppressed mice)
with E. bieneusi also have failed to date."
DIDIER, PETER J. (a veterinarian) <pdidier@tpc.tulane.edu>
"The objectives of this pilot project are to establish the
source of Mycobacterium avium infection in SIV-infected monkeys,
characterize immune correlates of disease, and determine the feasibility
of vaccination to prevent opportunistic infection of SAIDS animals
with M. avium. The incidence of M. avium infection (MAI) in the
SAIDS colony has increased from undetectable levels in 1988 to 18-25%
in recent years....We have demonstrated that animals infected with
SIV and severely immunodeficient can be infected with our pathogenic
strain (MavK128) while inoculation with a human strain of M. avium
(Serovar 4) fails to infect immunodeficient monkeys."
In another study, Peter Didier says: "The objectives of this
pilot project are to evaluate changes in ovarian morphology, serum
estrogen, bone density, and DNA deletions over time in female rhesus
monkeys. Preliminary results indicate that bone strength from young
monkeys 3-5 years of age and aged monkeys 22-25 years is different
and that breaking strength of femurs decreases substantially with
age."
HARRISON, RICHARD M. <harrison@tpc.tulane.edu>
"This is a continuation of services to other investigators
to provide pregnancies of known gestational ages and to provide
synchronized menstrual cycles in rhesus monkeys (Macaca mulatta).
For timed breeding, the monkeys? cycles are controlled by the daily
injection of progesterone (5 mg/day) for10 days. Females are paired
with males on days 14 to 21 after the last progesterone injection.
Ovulation and conception occurs on day 16 to 18 after the progesterone
injections. Success rate, based on viable fetus at 21 days gestation,
remains at 60% to 70% for first cycle matings. Fetal development
is monitored using ultrasonography. For synchronization of menstrual
cycles monkeys are examined twice daily to detect menses. On the
16th days of the cycle daily progesterone injections begin. Injections
are continued until all the monkeys to be synchronized have received
a minimum of 10 injections. At that point all injections cease.
Menses occurs on day 1 or 2 following the last progesterone inj
ection. If the investigator wishes to treat the monkeys in the luteal
stage of the cycle, progesterone injections can begin again on day
16 - 18 and the monkeys can be maintained in a luteal stage for
the study."
Few women and few infants (assuming that the monkeys are intended
to be models of humans) will be exposed to such a chemical bath.
Such unnatural conditions are likely to confound any experimental
data and thus render any conclusions meaningless.
In another project, Harrison writes: "Reports in the literature
suggest that soy-based [infant] formulas may be associated with
an increased incidence of goiter and thyroid disease." But,
in an experiment that could seem logical only to primate vivisectors
and to those who fund them, Harrison placed monkeys in two equal
groups. "The experimental group received 8mg/kg body weight
of genistein [a chemical found in soy believed to offer protection
from some forms of cancer] each Monday through Friday. Dosing continued
until day 155 of gestation, at which time a Cesarean section was
performed to collect the fetus and placenta. Blood was collected
from the maternal peripheral circulation twice weekly during the
study period. At delivery blood was collected from the maternal
peripheral circulation, the uterine veins, the ovarian veins, and
the fetal heart." He discovered more genistein in the blood
of the fetuses of mothers who had been given the genistein!
LACKNER, ANDREW A. <alackner@tpc.tulane.edu>
is continuing to study the monkeys he is killing with SIV.
LEVY, LAURA S. <llevy@tulane.edu>
"Since 1984, over 1200 necropsies on SIV-infected rhesus and
cynomolgus monkeys have been performed at the TRPRC. Lymphoid malignancies
[cancer] were detected in a proportion of SIV-infected animals."
She seems hopeful of producing more of these cancer infected monkeys.
MARTIN, LOUIS N., "SENIOR RESEARCH SCIENTIST,"
<martin@tpc.tulane.edu>
writes: "Regions where HIV infection is prominent frequently
overlap malaria endemic regions. However, the impact of coinfection
with HIV and malaria is not clear." What is abundantly clear,
however, is that in regions of the world where HIV is prominent,
almost no medical care is available. Those with HIV in these areas,
such as central Africa, will receive no benefit from martin's work.
It is doubtful whether anyone will other than Martin and those who
are funded through his grant.
Martin writes: "We inoculated a monkey with Plasmodium knowlesi.
57 days after SIV inoculation, a time when the SIV infection had
already caused a decrease in the percentage of CD4+ cells. Maximum
parasitemia (11.6%) occurred 9 days after malaria inoculation and
was treated by chloroquine. After treatment the hematocrit recovered
steadily through day 26, when malaria re-emerged and a single chloroquine
treatment was given. The parasitemia and accompanying decrease in
hematocrit rapidly resolved. Malaria re-emerged on day 46 and was
again treated. After this third recrudescence and treatment, the
parasite did not reappear, so the monkey was reinoculated with malaria.
Nine days later the parasite was detected but did not require treatment,
remaining a chronic low level infestation. The coinfection with
malaria did not affect the progress of SIV infection. The percentage
of cells expressing CD4, CD8, CD2, or CD20 did not differ in the
malaria-infected monkey compared to other monkeys inoculated with
SIV at the same time. No conclusions can be drawn based on 1 monkey,
but the feasibility of the SIV/malaria coinfection has been demonstrated.
An additional SIV-infected monkey was recently inoculated with malaria
327 days after SIV, a point when the percentage of CD4+ cells had
declined markedly. A naive control monkey was inoculated at the
same time. It will be interesting to see if the SIV-infected monkey
inoculated with malaria at a much later stage of SIV infection will
still be able to contain the parasite after 3 treatments, and to
see if the ability to control the parasite differs in the control
monkey. FUNDING NIH-N01-AI-65310 PUBLICATIONS NONE."
MARX, PRESTON A. <pmarx@bellsouth.net>
"We ... propose to examine several of the issues concerning
sexual transmission in a rhesus macaque model using both SIV and
SHIV chimeric viruses. Specifically, we will attempt to define the
identity of the first cell(s) infected by SIV in the vaginal and
cervical mucosa of macaques....we will also track the kinetics and
the pathway of SIV spread from the genital mucosa to the draining
(internal iliac) lymph nodes before systemic dissemination. In addition,
we will perform a series of experiments to examine directly the
issue of selective transmission. Mixtures of SIV variants with known
differences in their biological phenotypes will be inoculated both
mucosally and intravenously into macaques to determine whether virus
with a particular biological prop erty (e.g., macrophage-tropism)
will be preferentially selected in animals inoculated mucosally.
Similar experiments will also be performed using mixtures of SHIV
chimeric viruses...."
PHILIPP, MARIO T. <philipp@tpc.tulane.edu>
Sometimes, the abstracts found at the CRISP site are written by
persons other than those doing the experiments. Such is the case
here. It is clear that whomever wrote the abstract is enamored with
Dr. Philipp: "Abstract: DESCRIPTION (Adapted from the applicant's
abstract): This project, presented by an outstanding group of investigators
that includes Drs. M. T. Philipp as P.I. and M. S. Klempner, is
designed to complement and expand a human study already in course
directed to probe the efficacy of an antibiotic regimen designed
to treat chronic Lyme disease." Thirty monkeys will be killed
altogether in this study. It remains to be seen: If the study is
underway in humans, why use monkeys? If the therapy is successful
in humans, why kill monkeys?
TRAINA-DORGE, VICKI L. <vicki@tpc.tulane.edu>
"A large RSV vaccine study using a Praxis Biologics, formalin-inactivated
(FI) RSV vaccine was conducted. Twenty-one seronegative rhesus were
divided into seven groups of three monkeys each. Four groups (Gp)
received IM injections of the FI-RSV vaccine, ranging from high
(250ul) to low (50ul of a 1:25 dilution), in five-fold dilutions,
while Gp 5 received the Praxis prepared FI-sham vaccine. An independently
prepared, Prince FI-RSV vaccine was used to inoculate Gp6, IM. Finally,
Gp 7 was intranasally infected with live RSV. Twenty-one days later,
a booster vaccine was administered to the first six groups. Finally,
on day 56 post inoculation, a live RSV challenge was administered
to all 21 animals....On day 8 post challenge, the animals were necropsied.
VEAZEY, RONALD S. <veazey@tpc.tulane.edu>
"The objective of this proposal is to carefully define developmental
changes in the immunophenotypic composition and function of the
systemic and mucosal immune system from neonate to adult in normal
and simian immunodeficiency virus (SIV)-infected macaques. In this
model, SIV serves as a useful tool to determine which differences
between the immature and mature immune system are responsible for
the increased susceptibility to SIV."
In other words, Ron infects baby monkeys with SIV and then takes
notes as he watches them die.
WHELTON, PAUL K. <pwhelton@tulane.edu>
explains the overall vivisection program at the facility: "The
score (sic) of the research projects, although primarily
in the area of infectious diseases, covers a range of biomedical
disciplines. The proposed AIDS research will include studies of
vaccines, the interaction of the IDS virus with other infectious
agents, the pathogenesis of disease in the monkey including the
transfer of virus form the mother to the fetus, the role of alcohol
in the infectious process, and the role of the mucosal immune response.
Gene therapy studies will test strategies for correcting globoid
cell leukodystrophy, hemophilia B, cystic fibrosis, liver disease,
and as a treatment for AIDS. Other infectious disease research will
be done on Lyme borreliosis (antibiotic treatment and autoimmunity),
malaria (severe cerebral disease and disease during pregnancy)<
lymphatic filariasis, and pyelonephritis in diabetics. Other, non-infectious
disease research projects will be done on neuropeptides, aging,
opioids, and vascular injury with balloon catheters. In addition
to the research junction of the Center, this grant will support
breeding colonies of macaques. It will also provide support for
this Center to serve as a resource for investigators from other
institutions who have need to conduct research in non-human primates."
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